Hotspot

ndhF-rpl32

SSC · 109068-109873 · 806 bp

Back to Polystachya

Pi 0.0526

MarkerSeek score 78.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 806
variable_sites: 79
pi: 0.0526
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGGCCCTCTTATCTCTTCC	CTTTTTGACTGTCCCGTGGA	55.0	50.0	57.9	58.0	1003-1467 bp	0.6	74.8
ndhF-rpl32_p2	GGGCCCTCTTATCTCTTCCT	CTTTTTGACTGTCCCGTGGA	55.0	50.0	57.9	58.0	1002-1466 bp	0.6	74.8
ndhF-rpl32_p3	TACGAGCTCGGGTTATGGAA	CTTTTTGACTGTCCCGTGGA	50.0	50.0	58.2	58.0	980-1444 bp	0.6	73.8
ndhF-rpl32_p4	GGAAGTATCAACTGCACCGA	CTTTTTGACTGTCCCGTGGA	50.0	50.0	57.6	58.0	1404 bp	0.2	44.7
ndhF-rpl32_p5	AGGAAGTATCAACTGCACCG	CTTTTTGACTGTCCCGTGGA	50.0	50.0	57.6	58.0	1405 bp	0.2	44.6