Hotspot

trnN-GUU-ndhF

SSC · 108172-108974 · 803 bp

Back to Polystachya

Pi 0.156

MarkerSeek score 74.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 803
variable_sites: 166
pi: 0.156
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CAAAGTGAGCCCTTTCCCTT	TGCTCCCGGTTACTATGTCA	50.0	50.0	58.0	58.1	1197 bp	0.2	46.8
trnN-GUU-ndhF_p2	GCAAAAATGCGTTCCTCCTC	TGCTCCCGGTTACTATGTCA	50.0	50.0	58.0	58.1	1437 bp	0.2	46.7
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	TGCTCCCGGTTACTATGTCA	50.0	50.0	58.0	58.1	1475 bp	0.2	46.6
trnN-GUU-ndhF_p4	TATGCCGAGGTATTGAGGGT	TGCTCCCGGTTACTATGTCA	50.0	50.0	57.9	58.1	1308 bp	0.2	46.1
trnN-GUU-ndhF_p5	CGGCCGCAAAGATATTGAAC	TGCTCCCGGTTACTATGTCA	50.0	50.0	57.9	58.1	1638 bp	0.2	46.0