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clpP-psbB

LSC · 70442-71433 · 992 bp

Back to Polystachya

Pi 0.0443

MarkerSeek score 88.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 83
pi: 0.0443
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.0	58.2	1056-1172 bp	1.0	88.5
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	972-1088 bp	1.0	88.5
clpP-psbB_p3	TATACGTCAACCCAAGCTGC	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.0	58.2	1051-1167 bp	1.0	88.4
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	967-1083 bp	1.0	88.3
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.8	58.2	1002-1118 bp	1.0	87.5