Hotspot

trnF-GAA-trnV-UAC

LSC · 48285-49399 · 1115 bp

Back to Polystachya

Pi 0.125

MarkerSeek score 78.70

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 176
pi: 0.125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	428-1182 bp	1.0	90.0
trnF-GAA-trnV-UAC_p2	GGAAATGGTCGGGATAGCTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	474-1228 bp	1.0	89.1
trnF-GAA-trnV-UAC_p3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	471-1225 bp	1.0	88.8
trnF-GAA-trnV-UAC_p4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	470-1224 bp	1.0	88.8
trnF-GAA-trnV-UAC_p5	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	427-1181 bp	1.0	87.3