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ndhA

SSC · 122018-124204 · 2187 bp

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Pi 0.00441

MarkerSeek score 57.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2187
variable_sites: 28
pi: 0.00441
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	CCGCCAGGTTTTGTCAATTT	47.6	45.0	58.1	57.5	2408-2420 bp	1.0	68.8
ndhA_p2	TGAACCCAGTTACCATAGGGA	CCGCCAGGTTTTGTCAATTT	47.6	45.0	57.8	57.5	2411-2423 bp	1.0	67.7
ndhA_p3	CCCAGTTACCATAGGGAACA	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2407-2419 bp	1.0	63.1
ndhA_p4	TGAACCCAGTTACCATAGGG	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2411-2423 bp	1.0	63.1
ndhA_p5	ACCCAGTTACCATAGGGAAC	CCGCCAGGTTTTGTCAATTT	50.0	45.0	56.2	57.5	2408-2420 bp	1.0	63.1