Hotspot

atpB

LSC · 54524-56020 · 1497 bp

Back to Polyspora

Pi 0.00271

MarkerSeek score 52.90

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 12
pi: 0.00271
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CAATAGGGGCGTGATTTGGT	CGATCAACTTGCTATCGGACA	50.0	47.6	58.2	58.2	1684-1696 bp	1.0	69.7
atpB_p2	CAATAGGGGCGTGATTTGGT	CGATCAACTTGCTATCGGAC	50.0	50.0	58.2	56.4	1684-1696 bp	1.0	66.7
atpB_p3	GGGCGTGATTTGGTAATACG	CGATCAACTTGCTATCGGACA	50.0	47.6	57.0	58.2	1678-1690 bp	1.0	63.4
atpB_p4	AGGGGCGTGATTTGGTAATAC	CGATCAACTTGCTATCGGACA	47.6	47.6	58.1	58.2	1680-1692 bp	1.0	63.0
atpB_p5	CAATAGGGGCGTGATTTGGTA	CGATCAACTTGCTATCGGACA	47.6	47.6	58.1	58.2	1684-1696 bp	1.0	63.0