Hotspot

trnV-GAC-rps12

IRa · 140632-142475 · 1844 bp

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Pi 0.000934
MarkerSeek score 51.30
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1844
variable_sites
7
pi
0.000934
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1996-2001 bp 1.0 77.6
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1935-1940 bp 1.0 77.3
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1939-1944 bp 1.0 77.3
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1934-1939 bp 1.0 77.3
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1937-1942 bp 1.0 76.4