Hotspot

rpl32-trnL-UAG

SSC · 115549-116469 · 921 bp

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Pi 0.00487

MarkerSeek score 50.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 921
variable_sites: 13
pi: 0.00487
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCAACCGGA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	939-1033 bp	1.0	75.5
rpl32-trnL-UAG_p2	AGGGAAATCTCTTTCAACCGG	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	940-1034 bp	1.0	75.4
rpl32-trnL-UAG_p3	GGGAAATCTCTTTCAACCGGA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	932-1026 bp	1.0	75.0
rpl32-trnL-UAG_p4	AGGGAAATCTCTTTCAACCGG	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	933-1027 bp	1.0	75.0
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCAACCGGA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	937-1031 bp	1.0	74.9