Hotspot

ndhF-rpl32

SSC · 114705-115386 · 682 bp

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Pi 0.00725
MarkerSeek score 40.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
682
variable_sites
13
pi
0.00725
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCAAAAGGTATGATCCACGC TCCAAATTGTTTTACGAATACGCT 50.0 33.3 57.5 57.8 782-789 bp 1.0 44.1
ndhF-rpl32_p2 GCAAAAGGTATGATCCACGC TCCAAATTGTTTTACGAATACGC 50.0 34.8 57.5 56.4 782-789 bp 1.0 41.8
ndhF-rpl32_p3 GCAAAAGGTATGATCCACGC CCAAATTGTTTTACGAATACGCT 50.0 34.8 57.5 56.4 781-788 bp 1.0 41.8
ndhF-rpl32_p4 GCAAAAGGTATGATCCACGC TTCCAAATTGTTTTACGAATACGC 50.0 33.3 57.5 57.1 783-790 bp 1.0 41.8
ndhF-rpl32_p5 GCAAAAGGTATGATCCACGC CCAAATTGTTTTACGAATACGCTT 50.0 33.3 57.5 57.1 781-788 bp 1.0 41.8