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petA-psbJ

LSC · 64888-65938 · 1051 bp

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Pi 0.00393

MarkerSeek score 55.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 13
pi: 0.00393
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1166-1194 bp	1.0	78.8
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1167-1195 bp	1.0	78.7
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1133-1161 bp	1.0	78.5
petA-psbJ_p4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1134-1162 bp	1.0	76.2
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1161-1189 bp	1.0	76.0