Hotspot

ycf3-trnS-GGA

LSC · 46003-46885 · 883 bp

Back to Polyspora

Pi 0.00133

MarkerSeek score 55.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 4
pi: 0.00133
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	673-993 bp	1.0	74.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	623-943 bp	1.0	74.5
ycf3-trnS-GGA_p3	ATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.6	58.2	672-992 bp	1.0	71.8
ycf3-trnS-GGA_p4	TTTATCCGCGATCTAGGCAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.6	58.2	671-991 bp	1.0	71.8
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTG	47.6	50.0	58.0	58.5	625-945 bp	1.0	71.7