Hotspot

trnG-UCC-trnfM-CAU

LSC · 37963-38125 · 163 bp

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Pi 0.0116

MarkerSeek score 52.90

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 6
pi: 0.0116
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	236-242 bp	1.0	82.3
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	272-278 bp	1.0	81.9
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	230-236 bp	1.0	81.9
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCAGTTCTTCTCTTCAGCGC	50.0	50.0	57.8	57.9	293-299 bp	1.0	81.3
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	229-235 bp	1.0	81.3