Hotspot

trnK-UUU-rps16

LSC · 4292-5057 · 766 bp

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Pi 0.00628
MarkerSeek score 53.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
766
variable_sites
16
pi
0.00628
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 937-941 bp 1.0 72.3
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 938-942 bp 1.0 72.2
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 942-946 bp 1.0 72.2
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 940-944 bp 1.0 72.2
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGAAACT 55.0 47.6 57.9 57.9 949-953 bp 1.0 71.9