Hotspot

trnH-GUG-psbA

LSC · 77-456 · 380 bp

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Pi 0.0113
MarkerSeek score 52.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
380
variable_sites
12
pi
0.0113
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 505-541 bp 1.0 78.4
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 506-542 bp 1.0 78.2
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 506-542 bp 1.0 77.9
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC ACCGTGCTAACCTTGGTATG 50.0 50.0 58.2 57.6 507-543 bp 1.0 77.7
trnH-GUG-psbA_p5 AATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 50.0 55.0 57.7 58.3 498-534 bp 1.0 76.9