Hotspot

atpA-sufC

Genome · 176058-177140 · 1083 bp

Back to Polyopes

Pi 0.271

MarkerSeek score 62.30

Primer available yes

Label atpA-sufC

Feature Metadata

label_name: atpA-sufC
feature_type: igs
region: Genome
length_bp: 1083
variable_sites: 54
pi: 0.271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.18
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-sufC_p1	GAGGCCTATCCAGGAGATGT	AGCCGATTCTGGACTCGATA	55.0	50.0	58.0	58.0	1969-2217 bp	0.666667	70.4
atpA-sufC_p2	AGCAAGCACAAGCATATCGA	AGCCGATTCTGGACTCGATA	45.0	50.0	58.0	58.0	2025-2273 bp	0.666667	70.3
atpA-sufC_p3	TGAAACAGGTCGCAGGAAAA	AGCCGATTCTGGACTCGATA	45.0	50.0	58.0	58.0	1695-1943 bp	0.666667	70.2
atpA-sufC_p4	GAGGCCTATCCAGGAGATGT	CTAAAGTCATCGCTGGGCAT	55.0	50.0	58.0	58.0	2351-2599 bp	0.666667	70.2
atpA-sufC_p5	GAGGCCTATCCAGGAGATGT	TAAAGTCATCGCTGGGCATC	55.0	50.0	58.0	58.0	2350-2598 bp	0.666667	70.1