Hotspot

petA-tatC

Genome · 148963-149551 · 589 bp

Back to Polyopes

Pi 0.265

MarkerSeek score 76.10

Primer available yes

Label petA-tatC

Feature Metadata

label_name: petA-tatC
feature_type: igs
region: Genome
length_bp: 589
variable_sites: 121
pi: 0.265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-tatC_p1	GGTATGGGGCCTACAACAAG	AAATTTTTGGCACCAGCAGC	55.0	45.0	57.9	58.4	1407 bp	0.333333	49.7
petA-tatC_p2	GGTATGGGGCCTACAACAAG	CAGCTTGCTCCAGGTGAATA	55.0	50.0	57.9	57.6	1605 bp	0.333333	49.6
petA-tatC_p3	GCTTCAAAGACGCTGTTTGG	AAATTTTTGGCACCAGCAGC	50.0	45.0	58.2	58.4	1191 bp	0.333333	48.2
petA-tatC_p4	GCTTCAAAGACGCTGTTTGG	CAGCTTGCTCCAGGTGAATA	50.0	50.0	58.2	57.6	1389 bp	0.333333	48.1
petA-tatC_p5	TTATCTCCTGGTATGGGGCC	AAATTTTTGGCACCAGCAGC	55.0	45.0	58.3	58.4	1416 bp	0.333333	48.0