Hotspot

trnS-GCU-trnD-GUC

Genome · 98256-104471 · 6216 bp

Back to Polyopes

Pi 0.0965
MarkerSeek score 62.50
Primer available yes
Label trnS-GCU-trnD-GUC

Feature Metadata

label_name
trnS-GCU-trnD-GUC
feature_type
igs
region
Genome
length_bp
6216
variable_sites
320
pi
0.0965
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.48
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnD-GUC_p1 GCGATACCTTAACGTCTAGACA TCTTCTCTTTTGTCAAATAAACA 45.5 26.1 57.5 52.5 775 bp 0.333333 13.3
trnS-GCU-trnD-GUC_p2 TGCGATACCTTAACGTCTAGAC TCTTCTCTTTTGTCAAATAAACA 45.5 26.1 57.5 52.5 776 bp 0.333333 13.3
trnS-GCU-trnD-GUC_p3 GCGATACCTTAACGTCTAGACA TCTTCTCTTTTGTCAAATAAACAA 45.5 25.0 57.5 53.3 775 bp 0.333333 13.3
trnS-GCU-trnD-GUC_p4 TGCGATACCTTAACGTCTAGAC TCTTCTCTTTTGTCAAATAAACAA 45.5 25.0 57.5 53.3 776 bp 0.333333 13.3
trnS-GCU-trnD-GUC_p5 GCGATACCTTAACGTCTAGACA ATCTTCTCTTTTGTCAAATAAACA 45.5 25.0 57.5 53.1 776 bp 0.333333 13.3