Hotspot

rpl11-trnW-CCA

Genome · 86172-86849 · 678 bp

Back to Polyopes

Pi 0.173

MarkerSeek score 85.90

Primer available yes

Label rpl11-trnW-CCA

Feature Metadata

label_name: rpl11-trnW-CCA
feature_type: igs
region: Genome
length_bp: 678
variable_sites: 171
pi: 0.173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl11-trnW-CCA_p1	GAAAACGGCGCGAATGTTTA	GTCGAGGGTTCAAGTCCTTC	45.0	55.0	58.0	57.9	1143-1177 bp	0.666667	70.7
rpl11-trnW-CCA_p2	GAAAACGGCGCGAATGTTTA	CCTATCCCTGAAGCAGCAAG	45.0	55.0	58.0	58.0	1493-1529 bp	0.666667	70.5
rpl11-trnW-CCA_p3	TGCTTGTGCCAGTAGAACTG	CCTATCCCTGAAGCAGCAAG	50.0	55.0	58.1	58.0	1268-1304 bp	0.666667	70.5
rpl11-trnW-CCA_p4	GAAAACGGCGCGAATGTTTA	GATTCCGATGCACCCTATCC	45.0	55.0	58.0	57.9	1542 bp	0.333333	52.2
rpl11-trnW-CCA_p5	GGCGGCGTTTTTAGAACAAA	CCTATCCCTGAAGCAGCAAG	45.0	55.0	57.9	58.0	1279 bp	0.333333	52.1