Hotspot

rrn16-ompR

Genome · 67410-67940 · 531 bp

Back to Polyopes

Pi 0.139
MarkerSeek score 80.60
Primer available yes
Label rrn16-ompR

Feature Metadata

label_name
rrn16-ompR
feature_type
igs
region
Genome
length_bp
531
variable_sites
109
pi
0.139
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-ompR_p1 GAGCCAGGATCAAACTCTCC GCACTTACAACTTCGTAGCC 55.0 50.0 57.7 56.8 577-597 bp 1.0 76.8
rrn16-ompR_p2 TGCATGTGTTAGGCATACCG GCACTTACAACTTCGTAGCC 50.0 50.0 58.3 56.8 611-631 bp 1.0 76.7
rrn16-ompR_p3 GAGCCAGGATCAAACTCTCC GCACTTACAACTTCGTAGCCA 55.0 47.6 57.7 58.6 577-597 bp 1.0 73.4
rrn16-ompR_p4 GAGCCAGGATCAAACTCTCC TGCACTTACAACTTCGTAGCC 55.0 47.6 57.7 58.6 578-598 bp 1.0 73.4
rrn16-ompR_p5 TGCATGTGTTAGGCATACCG GCACTTACAACTTCGTAGCCA 50.0 47.6 58.3 58.6 611-631 bp 1.0 73.2