Hotspot

ycf22-psaC

Genome · 15938-17085 · 1148 bp

Back to Polyopes

Pi 0.113

MarkerSeek score 83.20

Primer available yes

Label ycf22-psaC

Feature Metadata

label_name: ycf22-psaC
feature_type: igs
region: Genome
length_bp: 1148
variable_sites: 191
pi: 0.113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22-psaC_p1	CGCTGCCCTGACTAAATCAT	TGGGTGTAAGCGTTGTGAAA	50.0	45.0	58.0	58.0	1551-1562 bp	0.666667	70.4
ycf22-psaC_p2	CGCTGCCCTGACTAAATCAT	TTGGGTGTAAGCGTTGTGAA	50.0	45.0	58.0	58.0	1552-1563 bp	0.666667	70.4
ycf22-psaC_p3	CGCTGCCCTGACTAAATCAT	TGCAGGTATGACAGGTGGTA	50.0	50.0	58.0	58.1	2179-2190 bp	0.666667	69.6
ycf22-psaC_p4	CGCTGCCCTGACTAAATCAT	GGACGTAACATAGGTGCAGG	50.0	55.0	58.0	58.1	2193-2204 bp	0.666667	69.5
ycf22-psaC_p5	CGCTGCCCTGACTAAATCAT	GCCAAGCAGGACGTAACATA	50.0	50.0	58.0	58.0	2201 bp	0.333333	52.9