Hotspot

ycf20-cpcG

Genome · 9223-9881 · 659 bp

Back to Polyopes

Pi 0.254

MarkerSeek score 84.60

Primer available yes

Label ycf20-cpcG

Feature Metadata

label_name: ycf20-cpcG
feature_type: igs
region: Genome
length_bp: 659
variable_sites: 244
pi: 0.254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf20-cpcG_p1	ACAACAAGAAGGTATGCAGCA	TATTGTGGCCAGGTCCTGTA	42.9	50.0	58.1	58.0	1474-1480 bp	0.666667	69.2
ycf20-cpcG_p2	ACAAGAAGGTATGCAGCATGT	TATTGTGGCCAGGTCCTGTA	42.9	50.0	57.9	58.0	1471 bp	0.333333	44.4
ycf20-cpcG_p3	ACAAGAAGGTATGCAGCATGT	GGCCAGGTCCTGTAAATCAG	42.9	55.0	57.9	58.0	1465 bp	0.333333	44.4
ycf20-cpcG_p4	ACAAGAAGGTATGCAGCATGT	ACAAAGGGTTTTAGGGCGAG	42.9	50.0	57.9	58.1	1728 bp	0.333333	44.0
ycf20-cpcG_p5	ACAACAAGAAGGTATGCAGCA	GGCCAGGTCCTGTAAATCAG	42.9	55.0	58.1	58.0	1468 bp	0.333333	43.9