Hotspot

ycf80-trnR-CCG

Genome · 6939-7708 · 770 bp

Back to Polyopes

Pi 0.104

MarkerSeek score 86.80

Primer available yes

Label ycf80-trnR-CCG

Feature Metadata

label_name: ycf80-trnR-CCG
feature_type: igs
region: Genome
length_bp: 770
variable_sites: 116
pi: 0.104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80-trnR-CCG_p1	TCAAGCCTTTTCGCCATACA	CTGGTGGTCGAAGGTTCAAA	45.0	50.0	57.8	58.0	1261 bp	0.333333	51.5
ycf80-trnR-CCG_p2	TCAAGCCTTTTCGCCATACA	ATCGGGCATGTAACTCAGTG	45.0	50.0	57.8	57.7	1303 bp	0.333333	49.3
ycf80-trnR-CCG_p3	TCAAGCCTTTTCGCCATACA	GATTCCGATTCTGGTGGTCG	45.0	55.0	57.8	58.4	1271 bp	0.333333	48.4
ycf80-trnR-CCG_p4	TCAAGCCTTTTCGCCATACA	ATTCTGGTGGTCGAAGGTTC	45.0	50.0	57.8	57.5	1264 bp	0.333333	48.0
ycf80-trnR-CCG_p5	TCAAGCCTTTTCGCCATACA	GATTCTGGTGGTCGAAGGTT	45.0	50.0	57.8	57.5	1265 bp	0.333333	48.0