Hotspot

ndhF-rpl32

SSC · 121127-122055 · 929 bp

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Pi 0.0425
MarkerSeek score 71.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
929
variable_sites
131
pi
0.0425
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGCTCCGATTAACAAAGGA CTATTGGCTATTGGGACCGG 50.0 55.0 58.0 57.8 1460-1612 bp 0.833333 73.1
ndhF-rpl32_p2 TCCCGCTCCGATTAACAAAG CTATTGGCTATTGGGACCGG 50.0 55.0 58.0 57.8 1462-1614 bp 0.833333 73.1
ndhF-rpl32_p3 CCGCTCCGATTAACAAAGGA TATTGGCTATTGGGACCGGA 50.0 50.0 58.0 58.2 1459-1611 bp 0.833333 73.1
ndhF-rpl32_p4 TCCCGCTCCGATTAACAAAG TATTGGCTATTGGGACCGGA 50.0 50.0 58.0 58.2 1461-1613 bp 0.833333 73.1
ndhF-rpl32_p5 CCGCTCCGATTAACAAAGGA TCGAAGTCCCGGTAGAAAGA 50.0 50.0 58.0 57.8 1129-1281 bp 0.833333 73.0