Hotspot

rpl33-rps18

LSC · 71545-72060 · 516 bp

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Pi 0.0627

MarkerSeek score 70.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 103
pi: 0.0627
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGTTCATGGCCAAGGGTAAA	ACTCGGATAAACGCAGCAAT	50.0	45.0	58.1	58.0	1310-1435 bp	1.0	91.3
rpl33-rps18_p2	TAAAAAGGGGGTTCATGGCC	ACTCGGATAAACGCAGCAAT	50.0	45.0	58.1	58.0	1412-1444 bp	0.833333	75.6
rpl33-rps18_p3	ATGCGGATCTAGGGCAAAAG	ACTCGGATAAACGCAGCAAT	50.0	45.0	58.0	58.0	1742-1774 bp	0.833333	75.3
rpl33-rps18_p4	GAAAATGCGGATCTAGGGCA	ACTCGGATAAACGCAGCAAT	50.0	45.0	58.0	58.0	1746-1778 bp	0.833333	75.3
rpl33-rps18_p5	TGAAAATGCGGATCTAGGGC	ACTCGGATAAACGCAGCAAT	50.0	45.0	58.0	58.0	1747-1779 bp	0.833333	75.3