Hotspot

ndhC-trnV-UAC

LSC · 53947-54488 · 542 bp

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Pi 0.0947

MarkerSeek score 69.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 170
pi: 0.0947
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCATAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	26.1	50.0	52.2	57.8	595-826 bp	1.0	49.8
ndhC-trnV-UAC_p2	TCATATTCATAAAGCAGAAACAT	GAAGGTCTACGGTTCGAGTC	26.1	55.0	52.2	57.5	594-825 bp	1.0	49.8
ndhC-trnV-UAC_p3	TCATATTCATAAAGCAGAAACAT	GTTCGAGTCCGTATAGCCCT	26.1	55.0	52.2	58.7	583-814 bp	1.0	49.8
ndhC-trnV-UAC_p4	TCATATTCATAAAGCAGAAACAT	AAGGTCTACGGTTCGAGTCC	26.1	55.0	52.2	58.8	593-824 bp	1.0	49.8
ndhC-trnV-UAC_p5	TCATATTCATAAAGCAGAAACAT	GAGAAGGTCTACGGTTCGAG	26.1	55.0	52.2	57.2	596-827 bp	1.0	49.8