Hotspot

trnF-GAA-ndhJ

LSC · 51599-52273 · 675 bp

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Pi 0.0577

MarkerSeek score 71.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 128
pi: 0.0577
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AACAAGATGCAGGGTCGTTT	50.0	45.0	58.1	58.0	1191-1247 bp	1.0	89.7
trnF-GAA-ndhJ_p2	ACAGGAAATTCCGGGACCTA	AAACAAGATGCAGGGTCGTT	50.0	45.0	58.0	58.0	1340-1342 bp	0.833333	73.4
trnF-GAA-ndhJ_p3	ACAGGAAATTCCGGGACCTA	AACAAGATGCAGGGTCGTTT	50.0	45.0	58.0	58.0	1339-1341 bp	0.833333	73.4
trnF-GAA-ndhJ_p4	TACAGGAAATTCCGGGACCT	AAACAAGATGCAGGGTCGTT	50.0	45.0	58.0	58.0	1341-1343 bp	0.833333	73.4
trnF-GAA-ndhJ_p5	TACAGGAAATTCCGGGACCT	AACAAGATGCAGGGTCGTTT	50.0	45.0	58.0	58.0	1340-1342 bp	0.833333	73.4