Hotspot

trnL-UAA-trnF-GAA

LSC · 51159-51525 · 367 bp

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Pi 0.0494

MarkerSeek score 69.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 61
pi: 0.0494
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	435-457 bp	1.0	87.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	397-419 bp	1.0	84.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	432-454 bp	1.0	84.5
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	424-446 bp	1.0	83.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	407-429 bp	1.0	83.4