Hotspot

trnT-UGU-trnL-UAA

LSC · 49995-50518 · 524 bp

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Pi 0.0632

MarkerSeek score 72.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 109
pi: 0.0632
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CCCCCAGACGTCGTATTTTT	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	57.9	58.0	1365-1689 bp	1.0	90.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	AGAAGGAGTTGGCTCAGGAT	55.0	50.0	57.9	58.0	682-1119 bp	1.0	89.6
trnT-UGU-trnL-UAA_p3	GAGTCGAACCGATGACCATC	AGAAGGAGTTGGCTCAGGAT	55.0	50.0	58.2	58.0	713-1150 bp	1.0	89.3
trnT-UGU-trnL-UAA_p4	CCCCCAGACGTCGTATTTTT	TAACAACGCAAGACCGTCAA	50.0	45.0	57.9	58.1	1447-1457 bp	0.833333	74.3
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TAACAACGCAAGACCGTCAA	55.0	45.0	57.9	58.1	764-773 bp	0.833333	73.6