Hotspot

rps4-trnT-UGU

LSC · 49332-49921 · 590 bp

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Pi 0.0676

MarkerSeek score 72.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 127
pi: 0.0676
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTAACTAATTGGCGGGCTCC	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	58.0	58.0	1642-1966 bp	1.0	91.3
rps4-trnT-UGU_p2	AAAACCTGACCTGTTGACCC	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	57.9	58.0	1555-1879 bp	1.0	91.2
rps4-trnT-UGU_p3	AATAGTCGGAGCCATACCCA	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	57.9	58.0	1619-1943 bp	1.0	90.9
rps4-trnT-UGU_p4	ATAGTCGGAGCCATACCCAA	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	57.9	58.0	1618-1942 bp	1.0	90.9
rps4-trnT-UGU_p5	CCCCCAGACGTCGTATTTTT	AGAAGGAGTTGGCTCAGGAT	50.0	50.0	57.9	58.0	1365-1689 bp	1.0	90.9