Hotspot

psbZ-trnG-GCC

LSC · 39146-39428 · 283 bp

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Pi 0.0767

MarkerSeek score 72.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 71
pi: 0.0767
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGGTACATCATTATGGATTGGA	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	383-431 bp	1.0	65.1
psbZ-trnG-GCC_p2	TCCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	384-432 bp	1.0	65.1
psbZ-trnG-GCC_p3	CCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	45.5	50.0	56.7	57.8	383-431 bp	1.0	63.5
psbZ-trnG-GCC_p4	TGGATTGGATTAGTCTTTCTGGT	CGTCTTCTCCTTGGCAAAGA	39.1	50.0	57.2	57.8	369-417 bp	1.0	59.7
psbZ-trnG-GCC_p5	TGGATTAGTCTTTCTGGTAGGT	CGTCTTCTCCTTGGCAAAGA	40.9	50.0	55.9	57.8	364-412 bp	1.0	57.3