Hotspot

psbM-trnD-GUC

LSC · 31427-32655 · 1229 bp

Back to Polygonum

Pi 0.0347

MarkerSeek score 69.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 140
pi: 0.0347
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTTACTTCCA	GTTCGAGCCCCGTCAGTC	35.0	66.7	52.0	60.1	1044-1272 bp	1.0	52.0
psbM-trnD-GUC_p2	ATGCGAGAATATTTACTTCCA	GTTCGAGCCCCGTCAGTC	33.3	66.7	52.7	60.1	1045-1273 bp	1.0	52.0
psbM-trnD-GUC_p3	TGCGAGAATATTTACTTCCAT	GTTCGAGCCCCGTCAGTC	33.3	66.7	52.7	60.1	1044-1272 bp	1.0	52.0
psbM-trnD-GUC_p4	AATGCGAGAATATTTACTTCCA	GTTCGAGCCCCGTCAGTC	31.8	66.7	53.6	60.1	1046-1274 bp	1.0	52.0
psbM-trnD-GUC_p5	AAATGCGAGAATATTTACTTCCA	GTTCGAGCCCCGTCAGTC	30.4	66.7	54.4	60.1	1047-1275 bp	1.0	52.0