Hotspot

petN-psbM

LSC · 30149-31321 · 1173 bp

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Pi 0.0407

MarkerSeek score 68.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 153
pi: 0.0407
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCCCTTTCGCTCGTTGTATG	GGAAGACGGTAGGGTGTAGA	50.0	55.0	58.0	57.9	1614-1653 bp	0.833333	73.3
petN-psbM_p2	TCCCTTTCGCTCGTTGTATG	AGGAAGACGGTAGGGTGTAG	50.0	55.0	58.0	57.9	1615-1654 bp	0.833333	73.3
petN-psbM_p3	GTTGATACGCGCTTGATTCG	GGAAGACGGTAGGGTGTAGA	50.0	55.0	58.0	57.9	2267-2306 bp	0.833333	73.2
petN-psbM_p4	GTTGATACGCGCTTGATTCG	AGGAAGACGGTAGGGTGTAG	50.0	55.0	58.0	57.9	2268-2307 bp	0.833333	73.2
petN-psbM_p5	CGTTGTATGGGGGAGAAGTG	GGAAGACGGTAGGGTGTAGA	55.0	55.0	58.0	57.9	1603-1642 bp	0.833333	73.2