Hotspot

trnC-GCA-petN

LSC · 29387-30058 · 672 bp

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Pi 0.0528

MarkerSeek score 73.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 118
pi: 0.0528
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCGGTTCAAATCTGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	728-877 bp	1.0	87.9
trnC-GCA-petN_p2	TTTCCCCGGTTCAAATCTGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	727-876 bp	1.0	82.0
trnC-GCA-petN_p3	TTTCCCCGGTTCAAATCTGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	736-885 bp	1.0	81.9
trnC-GCA-petN_p4	CCGGTTCAAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	723-872 bp	1.0	81.9
trnC-GCA-petN_p5	TTTCCCCGGTTCAAATCTGG	GACTACCATTAAAGCAGCCCA	50.0	47.6	58.1	58.0	743-892 bp	1.0	81.1