Hotspot

ndhF

SSC · 119744-121984 · 2241 bp

Back to Polygala

Pi 0.0408

MarkerSeek score 74.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 231
pi: 0.0408
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGCTTTGGGACGTTTACCA	TGTTCGAACTGAATCGTTCT	45.0	40.0	57.9	55.1	2948-2974 bp	0.285714	33.0
ndhF_p2	GGGACGTTTACCAGTGTCAG	TGTTCGAACTGAATCGTTCT	55.0	40.0	58.2	55.1	2941-2967 bp	0.285714	31.8
ndhF_p3	TTGGGACGTTTACCAGTGTC	TGTTCGAACTGAATCGTTCT	50.0	40.0	57.8	55.1	2943-2969 bp	0.285714	31.5
ndhF_p4	TTGCTTTGGGACGTTTACCA	TGTTCGAACTGAATCGTTCTT	45.0	38.1	57.9	55.9	2948-2974 bp	0.285714	31.4
ndhF_p5	TTGCTTTGGGACGTTTACCA	TTGTTCGAACTGAATCGTTCT	45.0	38.1	57.9	55.9	2949-2975 bp	0.285714	31.4