Hotspot

ndhE

SSC · 126484-126789 · 306 bp

Back to Polygala

Pi 0.0146

MarkerSeek score 45.10

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 15
pi: 0.0146
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGGCCAAAAACCAGTACTCA	TTCCCACTTTGTAGCTGCAG	50.0	50.0	58.0	58.1	1491-1508 bp	0.714286	74.8
ndhE_p2	GGGCCAAAAACCAGTACTCA	CGACCTTTGGAGACGGAATT	50.0	50.0	58.0	57.9	1375 bp	0.142857	44.9
ndhE_p3	TGGGCCAAAAACCAGTACTC	CGACCTTTGGAGACGGAATT	50.0	50.0	58.0	57.9	1376 bp	0.142857	44.9
ndhE_p4	TCCTCTGTTCTTGGAGCAGA	CGACCTTTGGAGACGGAATT	50.0	50.0	58.0	57.9	1158 bp	0.142857	44.8
ndhE_p5	ACCCATGCTTCGAGTTGTTT	CGACCTTTGGAGACGGAATT	45.0	50.0	58.0	57.9	1252 bp	0.142857	44.8