Hotspot

ndhG-ndhI

IRa · 127514-127792 · 279 bp

Back to Polygala

Pi 0.0711

MarkerSeek score 76.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: IRa
length_bp: 279
variable_sites: 49
pi: 0.0711
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CTGCAGCTACAAAGTGGGAA	TTGCTTTGGGACGTTTACCA	50.0	45.0	58.1	57.9	540-577 bp	0.714286	76.8
ndhG-ndhI_p2	CTGCAGCTACAAAGTGGGAA	AGATGGACTTTACCCAGGCT	50.0	50.0	58.1	58.0	1155-1193 bp	0.714286	72.4
ndhG-ndhI_p3	AATTCCGTCTCCAAAGGTCG	AGATGGACTTTACCCAGGCT	50.0	50.0	57.9	58.0	1271 bp	0.142857	44.7
ndhG-ndhI_p4	AATTCCGTCTCCAAAGGTCG	TTGCTTTGGGACGTTTACCA	50.0	45.0	57.9	57.9	656 bp	0.142857	44.5
ndhG-ndhI_p5	AATTCCGTCTCCAAAGGTCG	GGGACGTTTACCAGTGTCAG	50.0	55.0	57.9	58.2	649 bp	0.142857	43.3