Hotspot

ndhE-ndhG

SSC · 126790-126982 · 193 bp

Back to Polygala

Pi 0.101

MarkerSeek score 76.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 193
variable_sites: 47
pi: 0.101
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCTCTGTTCTTGGAGCAGA	TTCCCACTTTGTAGCTGCAG	50.0	50.0	58.0	58.1	1274-1305 bp	0.857143	81.8
ndhE-ndhG_p2	GGACAAGCGGATTCACATCT	TTCCCACTTTGTAGCTGCAG	50.0	50.0	58.0	58.1	1310-1341 bp	0.857143	81.6
ndhE-ndhG_p3	TCCTCTGTTCTTGGAGCAGA	CGACCTTTGGAGACGGAATT	50.0	50.0	58.0	57.9	1158 bp	0.142857	44.8
ndhE-ndhG_p4	GGACAAGCGGATTCACATCT	CGACCTTTGGAGACGGAATT	50.0	50.0	58.0	57.9	1194 bp	0.142857	44.7
ndhE-ndhG_p5	CATACGCATAAGAGAGGGCC	CGACCTTTGGAGACGGAATT	55.0	50.0	57.9	57.9	885 bp	0.142857	44.3