Hotspot

ccsA-ndhD

SSC · 124021-124341 · 321 bp

Back to Polygala

Pi 0.0789

MarkerSeek score 72.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 321
variable_sites: 61
pi: 0.0789
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATGAGGCATGGGGATCCTA	TGGACCGCGAGAGTTATTTG	50.0	50.0	57.9	58.0	668-690 bp	1.0	88.7
ccsA-ndhD_p2	TAATGAGGCATGGGGATCCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	57.9	58.0	669-691 bp	1.0	88.7
ccsA-ndhD_p3	GACTGCACAGTTATGGCTCA	TGGACCGCGAGAGTTATTTG	50.0	50.0	57.9	58.0	475-497 bp	0.857143	84.1
ccsA-ndhD_p4	GACTGCACAGTTATGGCTCA	GGACCGCGAGAGTTATTTGT	50.0	50.0	57.9	58.0	474-496 bp	0.857143	84.1
ccsA-ndhD_p5	TGAGGCATGGGGATCCTATT	TGGACCGCGAGAGTTATTTG	50.0	50.0	57.9	58.0	666-688 bp	0.857143	82.8