Hotspot

psbJ-petA

LSC · 58707-59725 · 1019 bp

Back to Polygala

Pi 0.0307
MarkerSeek score 74.30
Primer available yes
Label psbJ-petA

Feature Metadata

label_name
psbJ-petA
feature_type
igs
region
LSC
length_bp
1019
variable_sites
79
pi
0.0307
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-petA_p1 TGGCTCATATTCTGGATTGGG TGAGAAGGTTCAATTGTTCGA 47.6 38.1 57.5 55.5 1077-1104 bp 1.0 47.9
psbJ-petA_p2 GGCTCATATTCTGGATTGGGT TGAGAAGGTTCAATTGTTCGA 47.6 38.1 57.5 55.5 1076-1103 bp 1.0 47.9
psbJ-petA_p3 TGGCTCATATTCTGGATTGGG TGAGAAGGTTCAATTGTTCGAA 47.6 36.4 57.5 56.2 1077-1104 bp 1.0 47.9
psbJ-petA_p4 TGGCTCATATTCTGGATTGGG TTGAGAAGGTTCAATTGTTCGA 47.6 36.4 57.5 56.2 1078-1105 bp 1.0 47.9
psbJ-petA_p5 GGCTCATATTCTGGATTGGGT TGAGAAGGTTCAATTGTTCGAA 47.6 36.4 57.5 56.2 1076-1103 bp 1.0 47.9