Hotspot

trnQ-UUG-psbK

LSC · 15345-15980 · 636 bp

Back to Polygala

Pi 0.04
MarkerSeek score 79.60
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
636
variable_sites
64
pi
0.04
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CTCGAATAGCGGGACCAAAA ACCAATACAGATCAAACTAAAGA 50.0 30.4 58.0 53.4 709-719 bp 0.857143 43.6
trnQ-UUG-psbK_p2 TCGAATAGCGGGACCAAAAC ACCAATACAGATCAAACTAAAGA 50.0 30.4 58.3 53.4 708-718 bp 0.857143 43.6
trnQ-UUG-psbK_p3 CTCGAATAGCGGGACCAAAA ACCAATACAGATCAAACTAAAGAT 50.0 29.2 58.0 54.0 709-719 bp 0.857143 43.6
trnQ-UUG-psbK_p4 TCGAATAGCGGGACCAAAAC ACCAATACAGATCAAACTAAAGAT 50.0 29.2 58.3 54.0 708-718 bp 0.857143 43.6
trnQ-UUG-psbK_p5 AGTATGCTCTGGGACGGAAG ACCAATACAGATCAAACTAAAGA 55.0 30.4 58.9 53.4 739-749 bp 0.857143 43.2