Hotspot

atpE-trnM-CAU

LSC · 9338-9661 · 324 bp

Back to Polygala

Pi 0.0699

MarkerSeek score 76.60

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 52
pi: 0.0699
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	GATCCACAAGAAGCTCAGCA	GACATAGGTCTGCTTCTGGC	50.0	55.0	57.9	58.1	1121-1162 bp	0.857143	81.0
atpE-trnM-CAU_p2	TGATCCACAAGAAGCTCAGC	GACATAGGTCTGCTTCTGGC	50.0	55.0	57.9	58.1	1122-1163 bp	0.857143	80.9
atpE-trnM-CAU_p3	CATCGATGAAGCTACTGCGA	GCTCCATGTGCTCTGATTCA	50.0	50.0	57.9	58.0	1514-1549 bp	0.857143	79.8
atpE-trnM-CAU_p4	GATCCACAAGAAGCTCAGCA	GCTCCATGTGCTCTGATTCA	50.0	50.0	57.9	58.0	1196-1231 bp	0.714286	73.9
atpE-trnM-CAU_p5	TGATCCACAAGAAGCTCAGC	GCTCCATGTGCTCTGATTCA	50.0	50.0	57.9	58.0	1197-1232 bp	0.714286	73.9