Hotspot

trnH-GUG-psbA

LSC · 130-404 · 275 bp

Back to Polygala

Pi 0.0698

MarkerSeek score 72.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 44
pi: 0.0698
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATCCACTTGGCTACATCCG	TGTAGAAGCTCCATCTATAAATGG	52.6	37.5	56.9	55.8	291-323 bp	1.0	52.0
trnH-GUG-psbA_p2	ATCCACTTGGCTACATCCGC	TGTAGAAGCTCCATCTATAAATGG	55.0	37.5	60.2	55.8	291-323 bp	1.0	52.0
trnH-GUG-psbA_p3	CCACTTGGCTACATCCGC	TGTAGAAGCTCCATCTATAAATGG	61.1	37.5	58.2	55.8	289-321 bp	1.0	52.0
trnH-GUG-psbA_p4	ATCCACTTGGCTACATCCG	AGAAGCTCCATCTATAAATGG	52.6	38.1	56.9	52.5	288-320 bp	1.0	52.0
trnH-GUG-psbA_p5	ATCCACTTGGCTACATCCGC	AGAAGCTCCATCTATAAATGG	55.0	38.1	60.2	52.5	288-320 bp	1.0	52.0