Hotspot

rpl16

LSC · 83022-84433 · 1412 bp

Back to Poilannammia

Pi 0.00921

MarkerSeek score 64.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1412
variable_sites: 13
pi: 0.00921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTTTCCGTCGGTCTACCTG	ACAACAAACTCTTTGCTTTCAGT	50.0	34.8	57.6	57.6	1546-1549 bp	1.0	50.0
rpl16_p2	TTTTTCCGTCGGTCTACCTG	ACAAACTCTTTGCTTTCAGTCT	50.0	36.4	57.6	56.4	1543-1546 bp	1.0	48.4
rpl16_p3	TTTTCCGTCGGTCTACCTGT	ACAACAAACTCTTTGCTTTCAGT	50.0	34.8	58.7	57.6	1545-1548 bp	1.0	48.2
rpl16_p4	TTTTCCGTCGGTCTACCTGT	ACAAACTCTTTGCTTTCAGTCT	50.0	36.4	58.7	56.4	1542-1545 bp	1.0	46.6
rpl16_p5	TTTTTCCGTCGGTCTACCTG	ACAAACTCTTTGCTTTCAGTCTT	50.0	34.8	57.6	57.1	1543-1546 bp	1.0	45.9