Hotspot

ndhG

SSC · 120568-121098 · 531 bp

Back to Poilannammia

Pi 0.00942

MarkerSeek score 62.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 5
pi: 0.00942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGGATTTGATAAGAGCGAGA	CCTGGTCAGGTCAATAAAATCA	40.0	40.9	53.9	56.1	650 bp	1.0	40.6
ndhG_p2	AGAGCGAGAAAATTTCAATTTCGA	CCTGGTCAGGTCAATAAAATCA	33.3	40.9	57.8	56.1	639 bp	1.0	40.6
ndhG_p3	TGGATTTGATAAGAGCGAGAA	CCTGGTCAGGTCAATAAAATCA	38.1	40.9	54.8	56.1	650 bp	1.0	40.6
ndhG_p4	TGGATTTGATAAGAGCGAGA	CCTGGTCAGGTCAATAAAATCATG	40.0	41.7	53.9	57.9	650 bp	1.0	40.6
ndhG_p5	AGAGCGAGAAAATTTCAATTTCGA	CCTGGTCAGGTCAATAAAATCATG	33.3	41.7	57.8	57.9	639 bp	1.0	40.6