Hotspot

ndhD

SSC · 117894-119405 · 1512 bp

Back to Poilannammia

Pi 0.00331

MarkerSeek score 48.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1512
variable_sites: 5
pi: 0.00331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCATCTAGGCAAAATTTCAAGTCA	GAACGGGTTTTGGGGTCTAA	37.5	50.0	57.7	57.7	1661 bp	1.0	43.9
ndhD_p2	TCCATCTAGGCAAAATTTCAAGTC	GAACGGGTTTTGGGGTCTAA	37.5	50.0	57.5	57.7	1662 bp	1.0	41.9
ndhD_p3	CCATCTAGGCAAAATTTCAAGTCA	AGAACGGGTTTTGGGGTCTA	37.5	50.0	57.7	58.6	1662 bp	1.0	41.6
ndhD_p4	TCCATCTAGGCAAAATTTCAAGT	GAACGGGTTTTGGGGTCTAA	34.8	50.0	56.4	57.7	1662 bp	1.0	41.4
ndhD_p5	TCCATCTAGGCAAAATTTCAAGTC	AGAACGGGTTTTGGGGTCTA	37.5	50.0	57.5	58.6	1663 bp	1.0	40.2