Hotspot

trnW-CCA-trnP-UGG

LSC · 67633-67850 · 218 bp

Back to Poilannammia

Pi 0.0157
MarkerSeek score 64.10
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
218
variable_sites
3
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT AAGAAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 311-338 bp 1.0 84.2
trnW-CCA-trnP-UGG_p2 TTTTGGAGACCCACGTTCTG AAGAAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 310-337 bp 1.0 84.2
trnW-CCA-trnP-UGG_p3 TGCCGAACTGAACTAAGAGC AAGAAAGGGATGTAGCGCAG 50.0 50.0 57.9 58.0 292-319 bp 1.0 83.9
trnW-CCA-trnP-UGG_p4 ACCTACGACATCGGGTTTTG AAGAAAGGGATGTAGCGCAG 50.0 50.0 57.9 58.0 325-352 bp 1.0 83.8
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 271-298 bp 1.0 83.8