Hotspot

accD-psaI

LSC · 59958-60804 · 847 bp

Back to Poilannammia

Pi 0.00827

MarkerSeek score 63.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 7
pi: 0.00827
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	974-975 bp	1.0	66.8
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	973-974 bp	1.0	66.8
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	974-975 bp	1.0	65.0
accD-psaI_p4	AAAGGGTGTTCTGAGTGAGT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	981-982 bp	1.0	62.5
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	980-981 bp	1.0	62.5