Hotspot

rbcL-accD

LSC · 57863-58481 · 619 bp

Back to Poilannammia

Pi 0.0188

MarkerSeek score 70.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 10
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	CGTCCACGAGTGTCTTCAAT	50.0	50.0	57.9	57.9	721-789 bp	1.0	83.6
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CGTCCACGAGTGTCTTCAAT	55.0	50.0	57.7	57.9	737-805 bp	1.0	82.2
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	639-707 bp	1.0	82.1
rbcL-accD_p4	ACGTGAAGCTAGCAAATGGA	CGTCCACGAGTGTCTTCAAT	45.0	50.0	57.5	57.9	755-823 bp	1.0	80.7
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ACATCGAATTAAACCGCCGT	55.0	45.0	57.7	58.3	655-723 bp	1.0	80.7