Hotspot

ndhC-trnV-UAC

LSC · 51833-52566 · 734 bp

Back to Poilannammia

Pi 0.0152

MarkerSeek score 59.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 11
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	853-858 bp	1.0	79.0
ndhC-trnV-UAC_p2	CGGGGCTAAAATTCCGGAAA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.5	57.9	850-855 bp	1.0	77.3
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATTCCGG	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	854-859 bp	1.0	77.2
ndhC-trnV-UAC_p4	CGGGGCTAAAATTCCGGAAA	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.5	58.3	851-856 bp	1.0	75.4
ndhC-trnV-UAC_p5	TAATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	47.6	55.0	58.1	57.9	854-859 bp	1.0	72.4