Hotspot

trnY-GUA-trnE-UUC

LSC · 31391-31449 · 59 bp

Back to Poilannammia

Pi 0.102
MarkerSeek score 74.50
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
6
pi
0.102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CATTAACCGCTCGGGCATC AAGTATGCCCCCATCGTCTA 57.9 50.0 59.1 57.9 162 bp 1.0 65.7
trnY-GUA-trnE-UUC_p2 CATTAACCGCTCGGGCATC TAATATGATGGCGGTTGGGC 57.9 50.0 59.1 58.1 182 bp 1.0 65.4
trnY-GUA-trnE-UUC_p3 CATTAACCGCTCGGGCAT AAGTATGCCCCCATCGTCTA 55.6 50.0 57.9 57.9 162 bp 1.0 65.1
trnY-GUA-trnE-UUC_p4 CATTAACCGCTCGGGCAT TAATATGATGGCGGTTGGGC 55.6 50.0 57.9 58.1 182 bp 1.0 64.7
trnY-GUA-trnE-UUC_p5 CATTAACCGCTCGGGCATC TCGTCTAGTGGTTCAGGACA 57.9 50.0 59.1 57.7 149 bp 1.0 64.5